Phospho histone
WebAnti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) More>> MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References WebOct 8, 2014 · Cells expressing phosphorylated Histone-3 at Serine-28 are in the M phase and thus this protein can be used as a marker to screen M-phase cells. So immuno- fluorescence with phospho Histone-3...
Phospho histone
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WebAnti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB. Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells. WebFeb 22, 2024 · The phosphorylation of histone H3 at serine 10 (p-H3S10) has been shown to be closely correlated with mitotic chromosome condensation. We previously reported that intracellular silver nanoparticles (AgNPs) release Ag ions that alter actin filament dynamics, leading to the activation of Aurora kinases and the formation of p-H3S10 through a …
WebHistone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. [1] [2] Featuring a main globular domain and a long N-terminal tail, H3 is … WebApplication. Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence. Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected phospho-Histone H2A.
WebHistone phosphorylation occurs predominantly on serine, threonine, and tyrosine or residues through a phosphoester bond formation as well as on histidine, lysine, and arginine through phosphoramidate bonds [197]. Histone H2AX phosphorylation (γH2AX) at serine 139 was induced by ataxia telangiectasia-mutated (ATM) protein kinase, and is a key ... WebBecause phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection.
WebApr 11, 2024 · While all these data emphasize the oncogenic property of VprBP-mediated H2AT120p, it remains elusive whether phosphorylation of non-histone proteins also plays a critical role in VprBP-driven ...
WebConcerningab4270, Histone H1, phosphorylated PabRabbit x: Thisantibody is produced by using hyper-phosphorylated histone H1 as the immunogen. The number and location of … high waisted a line shorts costumeWebHistone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. how many eye floaters are too manyWebPhospho-histone H3 (PHH3) is a recently described immunomarker specific for cells undergoing mitoses. In this study, we evaluated the MI of 2 subsets of uterine smooth muscle tumors--6 leiomyosarcomas (LMS) and 6 smooth muscle tumors of undetermined malignant potential (STUMP)--using PHH3-labeled sections and hematoxylin and eosin … high waisted a line skirt gownH2AX becomes phosphorylated on serine 139, then called γH2AX, as a reaction on DNA double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The modification can happen accidentally during replication fork collapse or in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. γH2AX is a sensitive target for … high waisted a line shorts tuxedoWebPhospho-Histone H2A.X (Ser139) Antibody (14-9865-82) in ICC/IF Immunocytochemistry of fixed and permeabilized MCF7 cells treated for 30 minutes with 10 uM camptothecin and stained with 5 µg/mL of Anti-Human/Mouse phospho-H2AX (S139) Purified followed by 5 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI. Product Details high waisted a line skirt patternWebOur previous study demonstrated that PEDV downregulates histone deacetylase 1 (HDAC1) expression by binding viral nucleocapsid (N) protein to the transcription factor Sp1, inducing enhanced protein acetylation. ... We found that PEDV and its N protein inhibit STAT1 phosphorylation and nuclear localization via inducing STAT1 acetylation as a ... high waisted a line skirts longWebHuman Phospho-Histone H2AX (S139) Antibody Summary Species Reactivity Human Specificity Detects human Histone H2AX when phosphorylated at S139. Source Polyclonal Rabbit IgG Purification Antigen and protein A Affinity-purified Immunogen Phosphopeptide containing human Histone H2AX S139 site Formulation high waisted a line skirt outfits